Expression of L-CCR in HEK 293 cells reveals functional responses to CCL2, CCL5, CCL7, and CCL8

TitleExpression of L-CCR in HEK 293 cells reveals functional responses to CCL2, CCL5, CCL7, and CCL8
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Year2003
Estimated date2002-08-01
CategoryScientific Publication
PlatformNone
DescriptionAbstract: It has become clear in the past years that chemokines and chemokine receptors are pivotal regulators of cellular communication and trafficking. In addition to the 20 chemokine receptors that have been cloned and described, various orphan receptors with a chemokine receptor-like structure are known. We have investigated the orphan mouse chemokine receptor (L-CCR) in HEK 293 cells, a receptor that was originally described in a mouse macrophage cell line. Cells expressing this receptor show pertussis toxin-sensitive chemotaxis and small intracellular calcium transients in response to the chemokines CCL2, CCL7, CCL8, and CCL5. Biotinylated CCL2 binds to L-CCRexpressing cells, and transfection experiments with an L-CCR–green fluorescent protein fusion protein showed L-CCR expression in the membranes of recombinant HEK 293 cells. Although radioligand binding was not detected, it is suggested that LCCR is a functional chemokine receptor. J. Leukoc. Biol. 74: 243–251; 2003.
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